解剖学和形态学
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听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vec...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(03)00030-1
更新日期:2003-07-01 00:00:00
abstract::Based on hybridization studies, 21/32 multi-resistant clinical isolates of Acinetobacter baumannii contain class 1 integrons. Amplification products were obtained from 20 of the hybridization-positive strains. A single dfrA7 cassette was identified in 18 of the isolates and an integron with two cassettes (aadB-aadA4) ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(03)00011-8
更新日期:2003-03-01 00:00:00
abstract::We report herein the isolation and molecular characterization of pBGR1, the first native plasmid isolated from the genus Bartonella. Cloning and sequencing revealed a 2725-base pair (bp) cryptic plasmid comprising two open reading frames of considerable length, which were designated rep and mob. The regions containing...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00103-8
更新日期:2003-01-01 00:00:00
abstract::The repABC replicons have an unusual structure, since they carry genes coding for partitioning (repA, repB) and replication (repC) proteins, which are organized in an operon. So far, the presence of these compact bi-functional modules has been reported only in the megaplasmids of the Rhizobiaceae and within the plasmi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00100-2
更新日期:2002-09-01 00:00:00
abstract::We have modified an E. coli-staphylococcal shuttle vector for use in the general cloning and expression of genes from pathogenic staphylococci in Staphylococcus carnosus. As S. carnosus is non-pathogenic, this expression system will facilitate the study of the roles of individual gene products in the disease process. ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1016/s0147-619x(02)00004-5
更新日期:2002-05-01 00:00:00
abstract::Tools for mutagenesis and expression analyses are needed to study the role of bacterial genes. Here, we report the construction of pMH11, a small, mobilizable plasmid that replicates in Escherichia coli, but not in Azorhizobium caulinodans, a nodulating microsymbiont of Sesbania rostrata, and that contains a unique Ba...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2002.1565
更新日期:2002-03-01 00:00:00
abstract::A 50,709-bp cryptic plasmid isolated from a marine Micrococcus has been sequenced and found to contain a number of putative mobile genetic elements. The coding regions for 11 putative transposases comprise approximately 17% of the total plasmid sequence. The majority of these transposases are located within a 13-kb cl...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2001.1550
更新日期:2002-01-01 00:00:00
abstract::We report here the molecular characterization of pFNL10, a 3990-bp cryptic plasmid of Francisella novicida-like F6168. The plasmid was maintained in F. novicida Utah 112 and F. tularensis LVS strains. We sequenced the entire plasmid and found six open reading frames (ORFs)-ORF1, ORF2, ORF3, ORF4, ORF5, and ORFm. ORF3,...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2001.1548
更新日期:2001-11-01 00:00:00
abstract::Nucleotide sequence analysis of two cryptic plasmids, pRS2 (2544 bp) and pRS3 (3948 bp), from Oenococcus oeni revealed the presence in both of three major open reading frames with significant similarity to other small cryptic plasmids from O. oeni. The results suggest that those plasmids could be separated into two su...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2001.1537
更新日期:2001-09-01 00:00:00
abstract::DNA sequence polymorphism was determined for the microcin V gene cluster encoded on the microcin V plasmids of 12 natural isolates of Escherichia coli. These microcin V gene clusters are similar in DNA sequence, with only 10 of the 683 bp polymorphic. Further, the levels and patterns of microcin V gene cluster polymor...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2001.1525
更新日期:2001-07-01 00:00:00
abstract::Polymerase chain reaction (PCR) is one of the most powerful tools in cloning genes. For the direct cloning of PCR products, T-vectors, which contain complementary 3'-thymidine overhangs, are widely used. In the present study, we developed a plasmid, pNB-T, which was constructed by cloning an XcmI cassette with a suffi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1500
更新日期:2001-01-01 00:00:00
abstract::The complete sequence of the 10.9-kbp bacteriocinogenic plasmid pBL1 from Lactococcus lactis subsp. lactis IPLA 972 has been determined. Thirteen ORFs were encountered, of which 5 were incomplete. pBL1 proved to be a narrow-host-range plasmid which replicates neither in Bacilus subtilis nor in Lactobacillus spp. The s...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1482
更新日期:2000-11-01 00:00:00
abstract::A novel type I restriction-modification specificity subunit, S. LlaW12I, has been identified on the naturally occurring 8.0-kb plasmid pAW122 in the lactic acid bacterium Lactococcus lactis subsp. cremoris W12. Presence of the HsdS protein together with a complete type I restriction-modification system conferred incre...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1478
更新日期:2000-09-01 00:00:00
abstract::Small particles of metallic tungsten, known also as tungsten microprojectiles, are routinely used for biotechnological purposes. In such applications, tungsten was observed to affect the integrity of plasmid DNA. Here we present evidence that interaction between tungsten particles and intact circular plasmids pU19, pU...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1475
更新日期:2000-07-01 00:00:00
abstract::Plasmid RK2 codes for two species of the replication initiator protein TrfA (33 and 44 kDa). Both polypeptides are strongly associated with membrane fractions of Escherichia coli host cells (W. Firshein and P. Kim, Mol. Microbiol. 23, 1-10, 1997). We investigated the role of a 12-amino-acid hydrophobic region (HR) in ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.2000.1467
更新日期:2000-05-01 00:00:00
abstract::Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are thought to play a role in determining the frequency of conjugative transposition. The affinity of binding of a chimeric protein, which consisted of maltose binding protein fused to the carboxy-terminal DNA binding domain of Tn916 integra...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1440
更新日期:2000-03-01 00:00:00
abstract::Certain yeast strains secrete a protein toxin, which inhibits the growth of sensitive pathogens and yeasts. Studies have shown that production of the toxin is dependent on presence of linear, double-stranded DNA plasmids in the killer yeasts. In the yeast Pichia pastoris, two linear double-stranded DNA plasmids have b...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1452
更新日期:2000-03-01 00:00:00
abstract::An improved method for integration of exogenous DNA fragments at a defined site within the genome of Pseudomonas aeruginosa was developed. The method relies on two integration-proficient vectors, mini-CTX1 and mini-CTX2. These two vectors contain (1) a tetracycline (tet) selectable marker, (2) an oriT for conjugation-...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1441
更新日期:2000-01-01 00:00:00
abstract::The linear plasmid SCP1 of Streptomyces coelicolor A3(2) is one of the genetically more studied linear streptomycete replicons. Although the genetics of SCP1 and its interaction with the host chromosome have been analyzed for nearly three decades no information exists on its replication. With the help of an ordered co...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1419
更新日期:1999-11-01 00:00:00
abstract::Several staphylococcal plasmids (26-45 kb) carry all three streptogramin-resistance (Sg(R)) genes, vat, vgb, and vga. Seven such plasmids harbored by independent strains belonging to three taxa (Staphylococcus aureus, S. simulans, and S. cohnii subsp. urealyticum) were compared and the deleted derivative of one of the...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1412
更新日期:1999-09-01 00:00:00
abstract::Tn4555 is a 12.1-kb Bacteroides antibiotic resistance transposon representative of a novel class of transmissible genetic elements that can be transferred by resident conjugative tetracycline resistance transposons (Tc(r)-elements) but are not capable of self-transfer. Previously it was shown that Tn4555 transposes by...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1401
更新日期:1999-07-01 00:00:00
abstract::The two-hybrid system was used to show that the Rep proteins from three members of the Dictyostelium discoideum Ddp2 plasmid family, Ddp2, Ddp5, and Ddp6, form homomultimers but not heteromultimers when expressed in yeast cells. The results with deletion mutations suggest that multiple regions of the Rep proteins are ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1999.1399
更新日期:1999-05-01 00:00:00
abstract::A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restrictio...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1998.1380
更新日期:1999-01-01 00:00:00
abstract::An RP4-oriT shuttle vector pJIR1457 originally developed for Clostridium perfringens was successfully transferred by conjugation from Escherichia coli to Clostridium botulinum type A strains and to a nontoxigenic C. botulinum type A-transposon Tn916 mutant strain lacking the entire toxin gene cluster. The light chain ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1998.1366
更新日期:1998-11-01 00:00:00
abstract::A set of low copy number plasmid vectors for mammalian gene expression has been constructed. These vectors are derived from the previously described bacterial low copy number expression vectors, pWSK29 and pWKS30, which are present at six to eight copies per cell. The new plasmids also have the following useful proper...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1334
更新日期:1998-01-01 00:00:00
abstract::A 102-kb plasmid, pIJB1, was isolated from Burkholderia cepacia strain 2a, which is able to use 2,4-dichlorophenoxyacetate (2,4-D) as a sole carbon source, and a physical map of the plasmid has been established. It was observed that spontaneous loss of a 37-kb fragment of the plasmid after growth in nonselective mediu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1332
更新日期:1998-01-01 00:00:00
abstract::Sphingomonas sp. CFO6 (a member of the alpha group of Proteobacteria) was isolated from a Washington soil by enrichment on the insecticide carbofuran as a sole source of carbon and energy. This strain has been shown to harbor five plasmids, at least some of which are required for catabolism of carbofuran. Rearrangemen...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1289
更新日期:1997-01-01 00:00:00
abstract::Isolates of Rhodococcus equi from pneumonic foals possess an 85- or 90-kb virulence-associated plasmid. A prominent, thermoregulated surface antigen, VapA, encoded by these plasmids is thought to be important in virulence. A 135-kb fragment containing the origin of replication of R. equi strain 103 virulence-associate...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1311
更新日期:1997-01-01 00:00:00
abstract::A putative insertion sequence (IS1473) has been cloned and sequenced. The 1087-bp element was found between the moaA and the ndhA genes in the upstream region of the nicotine dehydrogenase (ndh) operon in the 160-kb pAO1 plasmid of Arthrobacter nicotinovorans. It is flanked by an imperfect repeat of 33 bp and carries ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.1272
更新日期:1997-01-01 00:00:00
abstract::Two distinct families of repetitive DNA elements (1.4 and 1.2 kb) were identified from S1 nuclease-treated genomic DNA of four strains of Thiobacillus ferrooxidans. The 1.4-kb fragment hybridized with IST2, an insertion sequence of T. ferrooxidans. The 1.2-kb fragment was cloned and sequenced. The sequence (IST445), 1...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1997.1306
更新日期:1997-01-01 00:00:00
abstract::Suggestions are made for the estimation of plasmid loss rates in bacterial populations that multiply by binary fission. The plasmid loss rate is defined as the probability of a division of a plasmid-carrying individual giving birth to one plasmid-free and one plasmid-carrying daughter cell. The unit of time is consequ...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0042
更新日期:1996-11-01 00:00:00
abstract::Transformation of Escherichia coli with Dictyostelium discoideum genomic DNA containing integrated shuttle vectors in multicopy, tandemly duplicated format resulted in the establishment of the linear plasmid molecules as circular monomeric replicons. The transformation efficiencies were comparable to those obtained wi...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0036
更新日期:1996-09-01 00:00:00
abstract::Brevibacterium linens RBL strain cryptic plasmid pRBL1 (8.0 kb) is described. A region involved in pRBL1 autonomous replication in Corynebacterium glutamicum was identified by insertion and deletion mapping and partially sequenced. This region encodes for a hypothetical 310-amino acid (aa) protein closely related to t...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0029
更新日期:1996-07-01 00:00:00
abstract::A total of 530 strains of rumen bacteria were screened for the presence of plasmid DNA. The percentage of plasmid-bearing strains was found to be the highest among the Bacteroides/ Prevotella group (9.9%), while it was less than 1% in the Butyrivibrio (0.2%) and Clostridium (0.6%) genera. A small cryptic plasmid pRAM4...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1996.0011
更新日期:1996-03-01 00:00:00
abstract::Recently an insertional mutagenesis procedure has been developed to permit cloning of genes affected in developmental mutants of Dictyostelium discoideum (Kuspa and Loomis, Proc. Natl. Acad. Sci. USA 89, 8803-8807, 1992). In this procedure a plasmid bearing the URA (pyr5-6) gene is linearized with a restriction enzyme...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.0003
更新日期:1995-11-01 00:00:00
abstract::Analysis of the 50-kb R-plasmid pTP10 from the clinical isolate Corynebacterium xerosis M82B revealed that the erythromycin resistance gene, ermCX, is located on a 4524-bp composite transposable element, Tn5432. The ends of Tn5432 are identical, direct repeats of an insertion sequence, designated IS1249, encoding a pu...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.9995
更新日期:1995-09-01 00:00:00
abstract::Upon excision, conjugative transposon Tn916 forms a nonreplicating circular intermediate (CI), which is essential both for transposition and conjugal transfer. In this work we developed an assay to quantify the circular forms of Tn916. By this method we defined a new measurable trait of Tn916-carrying strains: the "CI...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1032
更新日期:1995-07-01 00:00:00
abstract::Cloning vectors (pFD1001, pFD1192, pFD1194, and pFD1212) were constructed by extension of the host range of a 7.2-kb Rhizobium meliloti cryptic plasmid (pRM1132f) with the ColE1-based plasmids, pBR322, pACYC177, pACYC 184, pSUP301, or pHC179; mobilization was facilitated by introduction of the oriT region from pRK2, a...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1024
更新日期:1995-05-01 00:00:00
abstract::The effects of the single-strand origins (SSOs) of plasmid pMV158 on (i) the conversion of its single-stranded (ss) replication intermediates to double-stranded (ds) plasmid DNA and (ii) its maintenance were analyzed. The rolling-circle plasmid pMV158, which replicates via ssDNA intermediates, contains two single-stra...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1010
更新日期:1995-03-01 00:00:00
abstract::The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn3926, is presented. The sequence of Tn3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn21...
journal_title:Plasmid
pub_type: 杂志文章
doi:10.1006/plas.1995.1008
更新日期:1995-01-01 00:00:00